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1. Dissolve 1 g of salmon sperm DNA in loo ml of 0.4 M NAOH and stir OIN at room temp.
2. Place bottle in a boiling H20 bath for 45 minutes to shear DNA.
3. Chill on ice to at least easy to touch.
4. Neutralize with glacial acetic acid to pH 4-7.
5. Centrifuge to remove debris (approximately 30 min. or longer at 2500-3000 rpm).
6. Precipitate DNA with 2 vols ETOH and put at -200C for at least 1 hour or OIN.
7. Collect DNA by centrifgation
8. Rinse pellet 70% ETOH, dry briefly and dissolve in 50 ml (lX) TE (10 mM Tris-HC1, 1 mM EDTA, pH 7.5).
9. Check absorbance at 260 nm and dilute to desired concentration.
10. Aliquot DNA and store at -200C.
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