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| Dr. Park's Program ( BHNRC, ARS, USDA) is an unique research program to discover novel bio-active compounds from plants in order to prevent and treat various human diseases. Support and learn more about his program, click Dr. Park |
| Protocol |
FACS Protocol |
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| FACS Buffer:HBSS (w/o phenol red - Hank's BSS) 1% BSA 0.1% Sodium azide 1 mm EDTA (if adherent cells) |
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| FACS Protocol 1. Wash cells with FACS buffer - lx,06 cells in Falcon #2052 tubes. Spin 2K 4 mins. to pellet. 2. Pour off supernatant. Resuspend pellet in 10 ul primary antibody dissolved in FACS buffer. 3. Incubate on ice for 30 min. 4. Wash 2x with FACS buffer (1 ml each wash). Spin out cells. 5. Re-suspend pellet in 10 ul labeled secondary antibody in FACS buffer. 6. Wash 2x with FACS buffer. 7. Resuspend pellet in 0. 5 ml FACS buffer. Sample is now ready for analysis. |
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| FACS Protocol |
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If you want the protocol in PDF, contact Dr. Park (Your affiliation is required upon any request) |
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