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| Protocol |
Receptor In Situ |
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| Binding Solution: Labeled (125,) peptide or antibody (0.3 nM) in HBB |
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| Receptor In Situ Deparaf f inize tissues (6 uM on glass slides) in Xylene 2x Sit - Hydrate 2x 211 ea. 100% ethanol, then 70% ethanol - Dip into 3 mM KC1 (2.24 g/1) lx 111, then 2x 1011 ea., 40C - Add binding solution 200 ul/slide; 2 h, 12-150C - Wash in PBS pH 7.4 5x 111 ea. - Air dry using fan or cold hair dryer - Dip in emulsion (as per In-Situ protocols) - Store dessicated at 40C - Develop (as per In-Situ protocols) between 4 d and 15 d after initiation of experiment |
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| Van Obberghen-Schilling, E.E. et al., J. Clin. Invest. 68:1356-1365, 1981 |
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